Fale su'esu'e PCR

falesuesue PCR

1.Clean nofoa;2. pusaaisa vailaau;3. ta'avale fa'ama'i UV;4. Pusa pusaaisa maualalo;5. Ta'ele uamea;6. Centrifuge;7. Ta'elega vai;8. Nucleic extractor;9. Paipa;10. kapoti saogalemu;11. masini PCR;12. autoclave;13.Vortex mixer

eria oloa galuega faatino tele fa'ailoga Fa'ata'ita'iga
Vaega e saunia ai mea e fa'afefete Fa'amama nofoa Fa'atonu mea fa'afoliga 1 OLABO BBS-SDC
Centrifuge Centrifuge fa'ata'ita'iga 1 OLABO Mini-12
Vortex fa'afefiloi Faafefiloi le faataitaiga 1 OLABO 88882010
Ta'ele uamea Reagent faʻamavae ma faʻamafanafana 1 OLABO 88870005
Pipette Paipa 4 OLABO 0.5-10µl
10-100μl
20-200μl
100-1000μl
Tu'u paipa Tuu le paipa 1 OLABO Linear
O le pusaaisa maualalo maualalo Taofi reagents 1 OLABO BDF-25V270
pusaaisa vailaau Taofi reagents 1 OLABO BYC-310
Trolley fa'ama'i UV Avanoa fa'ama'i 1 OLABO MF-Ⅱ-ZW30S19W
Pusa felauaiga olaola Fa'ata'ita'iga felauaiga 1 OLABO QBLL0812
Nofoaga sauniuni mo fa'ata'ita'iga Kapoti saogalemu o meaola Fa'ata'ita'iga faiga 1 OLABO BSC-1500IIB2-X
Ta'ele vai Fa'ata'ita'iga le fa'agaoioia 1 OLABO HH-W600
Vortex fa'afefiloi Faafefiloi le faataitaiga 1 OLABO 88882010
Centrifuge Fa'ata'ita'iga centrifugation 1 OLABO TG-16W
TGL-16M
OLABO Mini-12
Pipette Paipa 4 OLABO 0.5-10µl
10-100μl
20-200μl
100-1000μl
Tu'u paipa Tuu le paipa 1 OLABO Linear
Nucleic Acid Extractor Aveese le nucleic acid 1 OLABO BNP96
O le pusaaisa maualalo maualalo Fa'ata'ita'iga teuina 1 OLABO BDF-86V348
pusaaisa vailaau Reagent teuina 1 OLABO BYC-310
Trolley fa'ama'i UV Avanoa fa'ama'i 1 OLABO MF-Ⅱ-ZW30S19W
vaega o le fa'alauteleina o au'ili'iliga masini PCR Fa'ata'ita'iga fa'ata'ita'iga fa'ateleina 1 OLABO MA-6000
O le pusaaisa maualalo maualalo Teu fa'ata'ita'iga 1 OLABO BDF-25V270
Trolley fa'ama'i UV Avanoa fa'amalama 1 OLABO MF-Ⅱ-ZW30S19W
Nofoaga fa'ama'i Autoclave Tu'umamaina o mea faigaluega tipitipi 1 OLABO BKQ-B75II
Mea fa'aaogaina fa'ata'ita'i Fautuaga Fa'aoga ile paipa E tusa ai ma manaoga moni OLABO TF-100-RS
TF-1000-RS
TF-300-RS
TF-200-RS
paipa PCR Fa'aaogā fa'atasi ma se meafaigāluega PCR e fa'amalamaina E tusa ai ma manaoga moni OLABO PCR-0208-C
PCR-2CP-RT-C
Fa'aau centrifuge Teu fa'ata'ita'iga fa'a'a'ai pe fa'aoga ile centrifuge E tusa ai ma manaoga moni OLABO MCT-150-C
Faiga fa'ata'ita'i Aoina faʻataʻitaʻiga 1 OLABO
Nucleic Acid Extraction Kit Fa'aaogā fa'atasi ma le su'ega nucleic acid E tusa ai ma manaoga moni OLABO
Mea fa'aaoga puipuia ufimata faafomai Meafaigaluega Puipui E tusa ai ma manaoga moni OLABO
suti puipui Meafaigaluega Puipui E tusa ai ma manaoga moni OLABO
'ofu tu'ufua fa'aaogā Meafaigaluega Puipui E tusa ai ma manaoga moni OLABO
Totini lima Meafaigaluega Puipui E tusa ai ma manaoga moni OLABO
'ava malosi Sapalai fa'ama'i pipisi E tusa ai ma manaoga moni OLABO 500ml
Vaila'au fufulu lima Sapalai fa'ama'i pipisi E tusa ai ma manaoga moni OLABO 500ml

falesuesue PCR

1. P2 potu sterile (e le aofia ai le potu fa'apolopolo) ua fa'apipi'iina i se seti o le fa'a'a'alili mama mama.

2. O le potu e teu ai mo le taimi e tasi le suiga o seevae ma mea timu, o le potu lona lua o laei, o le potu taele ma le potu faʻapipiʻi ua faʻapipiʻiina taʻitasi ma se faʻamavaeina faʻamama ea ma ili faʻafefe.

3. O le ea fou i fafo e ulufale atu i totonu o le potu komepiuta e ala i le faamama muamua, ona auina atu lea i le faamama maualuga (sapalai vent) i luga o le pito i luga o le potu mama e ala i le ea malulu, faʻaogaina o le vaʻaia, ma le ili faʻamalosi.E fa'aauupegaina ta'itasi ma le fetu'una'iga o le leo o le ea.Valve mo le tufatufaina atu o le ea ma le suiga o le eseesega o le mamafa.

4. O le ea ea i le pito o le ea faʻafefe faʻaogaina o le 0.3 mita i luga aʻe o le eleele, ma o loʻo i ai se vaʻa faʻatonutonu le ea mo le fetuunaiga o le ea.E tatau ona fa'apipi'i le paipa 'autu fa'atasi ma se potu e le toe fa'afo'i mai e taofia ai le tafe i tua o le ea fa'asu.
5. O le su'esu'ega o le vevela o lo'o tu'uina i totonu o le ala autu o le ea.